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KMID : 1094720130180040709
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 4 p.709 ~ p.714
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Continuous conversion of rice starch hydrolysate to 2-keto-D-gluconic acid by Arthrobacter globiformis C224
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Teng Wen-Hua
Sun Wen-Jing
Yu Bin
Cui Feng-Jie
Qian Jing-Ya
Liu Jing-Ze
Wang Liang
Qi Xiang-Hui
Wei Hua
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Abstract
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A continuous conversion process of rice starch hydrolysate to 2-keto-D-gluconic acid (2KGA) by Arthrobacter globiformis C224 was developed. Its feasibility for industrial application was also evaluated. Results showed that the initial cell concentration exceeding 1.25 g/L met the continuous 2KGA production at a stable dilution rate and media composition, while the dilution rate and feeding glucose concentration had a significant effect on 2KGA production performance. The optimal operating parameters were obtained as: 0.090/h of dilution rate and 171.0 g/L of feeding glucose concentration. Under these conditions, the steady state had a produced 2KGA concentration of 124.74 g/L, average volumetric productivity of 11.23 g/L/h, and yield of 0.97 g/g. In conclusion, continuous 2KGA production by the A. globiformis C224 strain would be a superior industrial process for the production of 2KGA in terms of its high 2KGA productivity and yield.
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KEYWORD
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2-keto-D-gluconic acid, Arthrobacter globiformis, rice starch hydrolysate, continuous fermentation
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